Fig. 2.
Gene expression analysis demonstrates isolation and enrichment of intestinal epithelial stem cells (IESCs), progenitors, and enteroendocrine cells by use of fluorescence-activated cell sorting (FACS). Semiquantitative RT-PCR conducted on FACSed NEG, subLO, LO, and HI cells demonstrates enrichment of IESC stem cell biomarkers in the LO populations (A), active Wnt/β-catenin and Notch1 signaling in subLO and LO populations (B), and enrichment of committed secretory progenitors in the subLO population and enteroendocrine cells in the HI population (C). Elevated lactase expression in the NEG population indicates this population is enriched for enterocytes. All data points represent means ± SE from 3 independent experiments; statistical analysis was by ANOVA and post hoc 2 sample t-tests were then performed. A P value <0.05 is considered statistically significant. Different letters above each bar represent data points that are statistically different from each other.