Table 1.
Primer sequences for quantitative real-time RT-PCR
Transcript | Primer | Rat (5’-3’ sequence) | Mouse (5’-3’ sequence) |
---|---|---|---|
HPRT | forward | CTCATGGACTGATTATGGACAGGAC | SAME |
reverse | GCAGGTCAGCAAAGAACTTATAGCC | ||
ND1 | forward | AAGCGGCTCCTTCTCCCTACAAAT | CCGGCCCATTCGCGTTATTCTTTA |
reverse | GCTCGATTTGTTTCTGCGAGGGTT | AACGGAAGCGTGGATAAGATGCTC | |
COX1 | forward | TCACTGCCAGTATTAGCAGCAGGT | n/a |
reverse | TCTGGGTGGCCGAAGAATCAGAAT | ||
LDHB | forward | CACGGGAGCTTATTTCTCCAGACT | TCGTGGTTTCCAACCCAGTGGATA |
reverse | GATTGCATCCACTTCCAATCACGC | ATTGCATCCGCTTCCAATCACACG | |
TPI | forward | GCTGCACAGAACTGCTACAAAGTG | SAME |
reverse | GTCTTCTTTCAGAGTGTCCCAGCA | ||
PDHC | forward | CATGGACACAGTATGAGCGATCCA | n/a |
reverse | GAGCTGCTTCCTCGACTTCTTTCT | ||
ANT2 | forward | ATGGGATTAACGGCCTGTACCAAG | SAME |
reverse | TATTCTTGGGATCCGGGAGCATTC |
HPRT, hypoxanthine-guanine phosphoribosyltransferase; ND1, complex I subunit ND1; COX1, cytochrome oxidase subunit 1; LDHB, lactate dehydrogenase B; TPI, triose phosphate isomerase; PDHC, pyruvate dehydrogenase complex E1 subunit; ANT2, adenine nucleotide translocator 2