FIGURE 7.
Localization of hGDH2 in the cytoplasm of Sertoli and Leydig cells in human testis by double immunofluorescence studies. Shown are images of paraffin-embedded formalin-fixed sections of human testis stained with 1) rabbit antiserum against hGDH2 (red staining), 2) mouse monoclonal antibody against vimentin (green staining), and 3) mouse monoclonal antibody against calretinin (green staining). In A, the rabbit antiserum was not preincubated, whereas in B, it was preincubated with the immunogenic peptide prior to staining. There was specific punctate immunoreactivity for hGDH2 in the cytoplasm of Sertoli cells (SC; A and C), which were labeled also with vimentin (D and E). This reactivity was almost completely abolished after preincubation of the rabbit antiserum with the immunogenic peptide (B). There was also hGDH2-specific staining in the cytoplasm of Leydig cells (LC) (F and H), which were also labeled with calretinin (G and H).