Fig. 1.
miR-33 and SREBP-2 are coexpressed. (A and B) The intragenic miR-33 is encoded within intron 16 of SREBP-2 (A), and both its sequence and genomic position are conserved across evolution (hsa, human; ptr, chimpanzee; mmu, mouse; rno, rat; bta, cow; gga, chicken; dme, fruit fly) (B). (C and D) Expression of miR-33 and selected SREBP-2 and LXR target genes in mouse (C) and human (D) primary macrophages following 48 h incubation in media containing low (closed bars) or high (open bars) levels of sterols (see Materials and Methods). **P < 0.01. Data are mean ± SD of three (human) or two (mouse) independent experiments in triplicate.