Figure 4.
Atlastin-2 and -3 localize to the ER. (A) HeLa cells were co-immunostained for endogenous atlastin-2 or -3 (green) and the cis/medial-Golgi marker GM130 (red). Merged images are to the right. (B) HeLa cells were transfected with VSVG-GFP (green) and fixed during the 40°C incubation to reveal the ER. Cells were then immunostained for endogenous proteins using antibodies specific for atlastin-2 and -3 (red). Merged images are at the right. Bars, 10 µm.