. 2010 Jul 6;16:1227–1240.

Table 1. Sequence of the primer used for RT–PCR amplification of selected genes to substantiate the results obtained by microarray.

Sample number	Gene name	Forward primer (5′→3′)	Reverse primer (5′→3′)	Product length (bp)
1	Vascular Endothelial Growth factor A (VEGF-A)	ATGCGGATCAAACCTCACC	ATCTGGTTCCCGAAACCCTG
	183			358
	165			304
	148			269
	121			172
2	Fibroblast Growth Factor 2 (FGF2)	GGAGAAGAGCGACCCTCAC	GTGCCACATACCAACTGGTG	221
3	(Angiopoietin 1 (Ang-1)	CCCAGAAACTTCAACATCTGG	GGACTGTGTCCATCAGCTC	537
4	Transforming growth factor, beta 1 (TGFB1)	GACTACTACGCCAAGGAGGTC	TCAACCACTGCCGCACAACTC	332
5	Nestin (NES)	CACCTGTGCCAGCCTTTCTTAA	CCACCGGATTCTCCATCCTTA	361
6	Neuron-specific class III beta-tubulin (Tuj1)	TCAAGCGCATCTCCGAGCAG	ACCGTAAAACGTCAGGCCTGGAG	444
7	Collagen 1 alpha 1	TCCCCAGCCACAAAGAGTCTA	TTTCCACACGTCTCGGTCA	201
8	Vimentin`	CAGGAACAGCATGTCCAAATCG	TGTACCATTCTTCTGCCTCCTGC	127
9	S100A2	GATCCATGATGTGCAGTTCTCT	GTTCTGCTTCAGGGTCGGT	310
10	RPL35A	GAACCAAAGGGAGCACACAG	CAATGGCCTTAGCAGGAAGA	236
11	PAX-6	GAATCAGAGAAGACAGGCCA	GGTAGGTATCATAACTCCG	302
12	S100A4	GATGAGCAACTTGGACAGCAA	CTGGGCTGCTTATCTGGGAAG	123
13	E-cadherin	AAGGTGACAGAGCCTCTGGATAGA	TCTGATCGGTTACCGTGATCAA	124
14	CD24	AACTAATGCCACCACCAAGG	CCTGTTTTTCCTTGCCACAT6	188
15	PBX-1	ACCCTTCGCCATGTTATCAG	ATTGCTGGGAGATCAGTTGG	189
16	OTX-1	CTCCACCCAGCTGTTAGCAT	CGCATGAAGATGTCAGGGTA	221
17	FOXA1	AGGGCTGGATGGTTGTATTG	AGGCCTGAGTTCATGTTGCT	150
18	SHC3	GACATCTACAGCACGCCAGA	CAAGGGCTGGTTCTTGAGAG	186
19	FOXF1	TTGGCAATATTTGCCGTGTA	CTGCACTCTAGCAGCCAAAA	209
20	CDH6	TCGAGAAAACAGGGAGCAGT	CGGTGGAGAAGATTCAGGAG	175
21	CDH11	GTGCCTGAGAGGTCCAATGT	GGGTAGGGCTGTTCTGATGA	165
22	Collagen VI alpha 1	ACAGTGACGAGGTGGAGATCA	GATAGCGCAGTCGGTGTAGG	122
23	Collagen IV alpha 2	TTGGCGGGTGTGAAGAAGTTT	CCTTGTCTCCTTTACGTCCCTG	178
24	IL-1B	GGGCCTCAAGGAAAAGAATC	TTCTGCTTGAGAGGTGCTGA	205
25	Fibronectin 1	GCAGTAACCACTATTCCTGCAC	TCCTGATACAACCACGGATGAG	192
26	T-cell differentiation protein 2 (MAL2)	TTGCCTCCTCCAATGTTCCTC	CAGTTAGCATCAATTTGAGCCAC	133
27	CTGF	CAGCATGGACGTTCGTCT	CCAACCACGGTTTGGTCCTT	117
28	SPARC (osteonectin)	CGAGACCTGTGACCTGGACAATG	TCCGGTACTGTGGAAGGAGTGG	127
29	Sflt-F	TGAGCACTGCAACAAAAAGG	TCCTCCGAGCCTGAAAGTTA	172
30	FLT-F	GGCTCTGTGGAAAGTTCAGC	GCTCACACACTGCTCATCCAAA	223
31	FGFR1, transcript variant 2 mRNA	TCCAGTGCATCCATGAACTCT	CTGTTGCGTCCGACTTCAA	268
32	Brain derived nerve growth factor	GATGCTCAGTAGTCAAGTGCC	GCCGTTACCCACTCACTAATAC	168
33	Chemokine (C-C motif) ligand 2	CAGCCAGATGCAATCAATGCC	TGGAATCCTGAACCCACTTCT	190
34	Chitanase 3 like 1	GAAGAGGCCCTGTCTAGGTA	AGATGATGTGGGTACAGAGG	250
35	Matrix Metallo proteinase 2 (MMP2)	CCGTCGCCCATCATCAAGTT	CTGTCTGGGGCAGTCCAAAG	169
36	Interleukin 1 aplha (IL-1A)	TGTGACTGCCCAAGATGAAG	CGCCTGGTTTTCCAGTATCT	238
37	Decorin	AGTTGGAACGACTTTATCTGTCC	GTGCCCAGTTCTATGACAATCA	160
38	Neurotrophin tyrosine kinase receptor 2	GATAAGCTGGACTCGGCACG	GGACGACATCCCTAGCAGCC	152
39	Connexin 43	TGTCCTTAAGTCCCTGCTAA	GTAGCTGAGGAATGATGAAAAAG	245