FIGURE 7.
Early proliferative response of psoriatic CD4+CD25− Tresp cells. A, Magnetic bead-sorted CD4+CD25− Tresp cells from normal volunteers and psoriatic patients were labeled with CFSE and cultured with allogeneic APCs. After 3 days, cells were harvested, stained with anti-CD4 mAb, and analyzed by flow cytometry. Up to three divisions were observed in the case of psoriatic CD4+CD25− Tresp cells, whereas normal CD4+CD25− Tresp cells divided only once. A representative result of three independent experiments (for both normal and psoriatic individuals) is shown. B, The percentage of proliferating populations of total CFSE-labeled CD4+CD25− T cells. Data represent the average results from three independent experiments for both normal and psoriatic individuals. Results are expressed as the mean ± SEM.