Table 2.

Age-related changes in expression of selected genes between two identified cholinergic neurons: R2 and LPl1.

Differentially expressed Aplysia transcript related to aging	GeneName on array/accession no	Evalue (human)	Fold change
	GeneName on array/accession no	Evalue (human)	QRT-PCR	Young R2	Young LPl1	Old R2	Old LPl1
AGING RELATED
Programmed cell death 8	HM05452 4	e-8				34*
Inhibitor of apoptosis	GU255952	e-28	80			10*	4
Insulin-like growth factor II	DQ479393	0.025				12
TRANSPORT
Kinesin 3	HM054522	e-39		8*
Major vault protein	GU255949	e-43		7*
Kinesin II	DQ539618.1	e-65		5*
DISEASE
Amyloid precursor protein (APP)	AY535409	e-39	5				5
Huntingtin	GU255950	e-40				3
CYTOSKELETON
Matrilin	HM054523	–				20*
Gelsolin	FJ210649	e-52	6, 6.5	5	4.5
beta-1 tubulin	AAP13560.1	0		3	3
BIOENERGETICS
Mitochondrial ribosomal protein S10	HM054525	e-29				3.4
ION CHANNELS
Potassium channel subunit	AAA27756.1	0				17*
Tandem pore potassium channel	AAZ94722.1	e-38		5*
Glutamate receptor GluR5	AAP41207	e-177		4	3.5
NON CODING RNA
451	AF401632.1	–		6*
PEPTIDES
Buccalin precursor	P20481	–		4	4.5
Abdominal ganglion L5-67	P07712	–		3.4	3.4
Tolloid-BMP-1 like	T31069	e-109				2.4	2.7
Feeding circuit activating peptide	AY118084.1	–					5
Pleurin	AAV84470	–		3.5	3.0
MIP-related peptide	AF160191.1	–					6

Fold changes from microarray analysis are correlated to the specific cell and age. Relative expression is measured by QRT-PCR.

*Data from the direct comparison arrays of young and old R2 (AAA array).