Figure 3.
mtm disrupts hemocyte distribution and recruitment to larval wounds. (A–C) mtm function did not affect the number of bled hemocytes (A), percentage of dead or dying hemocytes (B; see Materials and methods; genotypes as shown), or number of sessile hemocytes per segment of third instar larva (C). (D) GFP+ hemocytes imaged in dorsal segment: control (top), mtm RNAi (middle), and mtm expression (bottom). Boxes represent the higher magnified views at right. (E) GFP+ hemocytes (arrows) recruited 6 h after wounding to puncture sites in dorsal segments. (F) Either mtm depletion or expression reduced the number of hemocytes within one-wound diameter. (A and C–F) IR-mtm/+ or mCherry:mtm/+ with Pxn-GAL4, UAS-GFP/+. Error bars indicate SEM.