Table 2.
Summary of in vivo microdialysis publications
| Reference | Membrane (type/cut-off/length) | Flowrate; sampling interval | Species | Region | Method | AEA= | 2-AG= | 2-AG/AEA< |
|---|---|---|---|---|---|---|---|---|
| Giuffrida et al. (1999) | PC, 20 kDa, 4 mm | 10 µL·min−1; 30 min | Rat | DStr | GC-MS | 5 nM per 1.5 pmol | Not determined | – |
| Walker et al. (1999) | RC, 40 kDa, 2 mm | 1 µL·min−1; 15–30 min | Rat | PAG | LC-MS | 0.19 nM per 2.8 fmol | Not determined | – |
| Caille et al. (2007) | PES, 15 kDa, 2 mm | 0.6 µL·min−1; 10 min | Rat | NAcc | LC-MS | 2.2 nM per 0.44 fmol | 5.3 nM per 1.1 fmol | 2.4 |
| Ferrer et al. (2007) | PES, 15 kDa, 2 mm | 0.6 µL·min−1; 10 min | Rat | NAcc | LC-MS | 2.0 nM per 0.40 fmol | Not determined | – |
| Bequet et al. (2007) | PES, 6 kDa, 2 mm | 1 µL·min−1; 30 min | Rat | HTh | LC-MS/MS | 0.12 nM per 3.7 fmol | 0.66 nM per 19.7 fmol | 5.5 |
| Alvarez-Jaimes et al. (2009b) | PES, 15 kDa, 2 mm | 0.6 µL·min−1; 10 min | Rat | NAcc | LC-MS | 2.8 nM per 0.56 fmol | 5.6 nM per 1.1 fmol | 2.0 |
| Alvarez-Jaimes et al. (2009a) | PES, 15 kDa, 3 mm | 0.6 µL·min−1; 10 min | Rat | PFC | LC-MS | 1.4 nM per 0.28 fmol | 8.5 nM per 1.7 fmol | 6.1 |
| Orio et al. (2009) | PES, 15 kDa, 2 mm | 0.6 µL·min−1; 10 min | Rat | NAcc | LC-MS | 2.3 nM per 0.46 fmol | 10.3 nM per 2.1 fmol | 4.5 |
| Villanueva et al. (2009) | PES, 15 kDa, 1 mm | 0.6 µL·min−1; 10 min | Rat | POA | LC-MS | 1.0 nM per 0.2 fmol | 3.2 nM per 0.64 fmol | 3.2 |
| Long et al. (2009) | PES, 15 kDa, 1 mm | 0.6 µL·min−1; 10 min | Mouse | NAcc | LC-MS | 0.56 nM per 0.11 fmol | 4.4 nM per 0.88 fmol | 7.9 |
=
Dialysate eCB content listed as concentration (nM) and amount per sample (pmol or fmol).
<
Ratio of 2-AG to AEA concentration in the dialysate sample.
Membrane: PC, polycarbonate; RC, regenerated cellulose; PES, polyethylsulfone.
Region: DStr, dorsal striatum; PAG, periaqueductal grey; NAcc, nucleus accumbens; HTh, hypothalamus; PFC, prefrontal cortex; POA, preoptic area of the thalamus.
2-AG, 2-arachidonoylglycerol; AEA, anandamide, N-arachidonoyl-ethanolamine; eCB, endocannabinoid; GC-MS, gas chromatography – mass spectrometry; LC-MS, liquid chromatography – mass spectrometry.