Table 2.
mRNA expression of α2-adrenoceptor subtypes in FACS-isolated cell fractions from 3 separate dissociates obtained from the adult murine brain, determined by microarray analysis of 20 ng of total RNA.
Gene symbol |
Receptor | Astrocyte | NG2+ cell | Microglia | Endothelial | Thy1+ Neuron |
---|---|---|---|---|---|---|
Adra2a | α2A | Present Present Present |
Present Present Present |
Present Present Absent |
Present Maybe Absent |
Absent Present Absent |
Adra2b | α2B | Consistently absent |
Consistently absent |
Consistently absent |
Consistently absent |
Consistently absent |
Adra2c | α2C | Consistently absent |
Consistently absent |
Consistently absent |
Consistently absent |
Consistently absent |
The Table indicates Present call, Absent call and Maybe call. For a gene to be unequivocally present 3 Present calls are normally expected. Two Present calls have been interpreted as tentatively present, partly based on comparison with literature data, whereas a single Present call at most indicates questionable expression.