Figure 6.
Effect of SKF on native T-type currents in cerebellar PCs. (A) Representative T-type current during 200 ms depolarizations to submaximal test potentials (as described in Methods) before, after perfusion of 30 µM SKF and after wash-out (as indicated). Currents were elicited by 200 ms depolarizations to −45 mV every 10 s from a holding potential of −75 mV (with a 500 ms prepulse to −90 mV to remove inactivation, as described in Methods). The apparent rates of channel activation and inactivation did not vary significantly before or during perfusion of SKF (for statistics, see text). (B) Representative time-course of T-type current inhibition by 30 µM SKF and subsequent wash-out with control solution. (C) Mean time-course of T-type current inhibition by 30 µM SKF (n = 5). The perfusion of 30 µM SKF inhibited 60.1 ± 1.8% of the T-type current during ∼8 min of SKF application.