Table 1.

PfCRT Mutations and CQ Response Phenotypes in P. falciparum Lines from Different Geographical Regions

	Region	Line	72	74	75	76	97	220	271	326	356	371
Chloroquine-sensitive lines	PNG	D10	C	M	N	K	H	A	Q	N	I	R
All regions	Thailand	T2/C6	C	M	N	K	H	A	Q	N	I	R
	Malaysia	Camp/A1	C	M	N	K	H	A	Q	N	I	R
	Sudan	105/7^a	C	M	N	K	H	A	Q	N	I	R
	Sudan	106/1^a	C	I	E	K	H	S	E	S	I	I
	Sudan	REN	C	M	N	K	H	A	Q	N	I	R
	South Africa	FAB9	C	M	N	K	H	A	Q	N	I	R
	Kenya	K39^a	C	M	N	K	H	A	Q	N	I	R
	Kenya	M24^a	C	M	N	K	H	A	Q	N	I	R
	Liberia	LF4/1	C	M	N	K	H	A	Q	N	I	R
	The Netherlands	NF54^a	C	M	N	K	H	A	Q	N	I	R
	Sierra Leone	SL/D6	C	M	N	K	H	A	Q	N	I	R
	Mali	BC5	C	M	N	K	H	A	Q	N	I	R
	Mali	M5	C	M	N	K	H	A	Q	N	I	R
	Haiti	Haiti	C	M	N	K	H	A	Q	N	I	R
	Honduras	HB3^a	C	M	N	K	H	A	Q	N	I	R
Chloroquine-resistant lines	Indochina	Dd2^a	C	I	E	T	H	S	E	S	T	I
Southeast Asia and Africa	Vietnam	V1/S	C	I	E	T	H	S	E	S	T	I
	Thailand	TM284	C	I	E	T	H	S	E	S	T	I
	SE Asia	ItG2F6	C	I	E	T	H	S	E	S	I	I
	SE Asia	FCB	C	I	E	T	H	S	E	S	I	I
	SE Asia	R29	C	I	E	T	H	S	E	S	I	I
	Cambodia	Jcl	C	I	E	T	H	S	E	S	T	I
	Sudan	102/1^a	C	I	E	T	H	S	E	S	T	I
	South Africa	RB8	C	I	E	T	H	S	E	S	I	I
	South Africa	RB20	C	I	E	T	H	S	E	S	I	I
	Uganda	PAR^a	C	I	E	T	H	S	E	S	I	I
	Kenya	KMWII	C	I	E	T	H	S	E	S	I	I
	Mali	M2^a	C	I	E	T	H	S	E	S	I	I
	Mali	S35CQ^a	C	I	E	T	H	S	E	S	I	I
	Saõ Tomé	Cai	C	I	E	T	H	S	E	S	I	I
South America	Brazil	7G8^a	S	M	N	T	H	S	Q	D	L	R
	Brazil	DIV30^a	S	M	N	T	H	S	Q	D	L	R
	Brazil	ICSN66	S	M	N	T	H	S	Q	D	L	R
	Brazil	ECP^a	S	M	N	T	H	S	Q	D	L	R
	Brazil	PAD	S	M	N	T	H	S	Q	D	L	R
	Brazil	ItD12	S	M	N	T	H	S	Q	D	L	R
	Ecuador	Ecu1110	C	M	N	T	H	S	Q	D	L	R
	Colombia	Jav^a	C	M	E	T	Q	S	Q	N	I	T
	Colombia	IAJ^a	C	M	E	T	Q	S	Q	N	I	T

Complete pfcrt coding sequences for these lines were determined directly from RT-PCR amplified products. For all other lines, point mutations were determined from genomic DNA PCR products.