Table 2.
Tumor-initiating capacity of sorted pancreatic cancer cells in NOD/SCID and NSG mice. The TIC frequency was calculated using a limiting dilution analysis (with 95% confidence intervals) and significance determined by chi-squared analysis.(Hu and Smyth, 2009). See Figure S1 for gating strategy of sorted cells and histology of tumors.
Tumor formation | ||||||||
---|---|---|---|---|---|---|---|---|
Cell Phenotype | Mouse | Xenograft | 5,000 | 1,000 | 500 | 100 | TIC frequency−1 (95% CI) | P- value |
ALDH+ | NSG | Panc253 | 4/4 | 3/4 | 1/4 | 300 (190–500) | 0.471 | |
Panc219 | 4/4 | 3/4 | 2/4 | |||||
Panc140 | 4/4 | 2/4 | 2/4 | |||||
NOD/SCID | Panc253 | 4/4 | 4/4 | 2/4 | 330 (200–530) | |||
Panc219 | 4/4 | 3/4 | 2/4 | |||||
Panc140 | 3/4 | 1/4 | 0/4 | |||||
CD44+CD24+ | NSG | Panc253 | 4/4 | 3/4 | 240 (130–450) | 0.435 | ||
Panc219 | 2/2 | 3/4 | 1/4 | |||||
Panc140 | 1/2 | 1/3 | ||||||
NOD/SCID | Panc253 | 4/4 | 3/4 | 180 (100–330) | ||||
Panc219 | 2/2 | 3/4 | 4/4 | |||||
Panc140 | 1/2 | 1/3 |