Figure 4.
Microglia in connectives treated with isotonic potassium gluconate (KGlu) and the effect of 10 µM CBX. (A) A representative tracing of currents (bottom traces) from an oocyte injected with Hminx2 and voltage clamped at negative potentials with shifts to positive potentials (top traces). The oocyte was treated with oocyte saline in which 50% NaCl was replaced with potassium gluconate (KGlu) to open the innexon channels and with 100 µM CBX to close them. A CBX-sensitive current increased severalfold in KGlu, reflecting increased membrane conductance due to innexon channel opening. (B) Cell nuclei stained with Hoechst 33258 dye were imaged using time-lapse microscopy, and microglia counted moving 25 µm for 30 min beginning at 30 min. The leech Ringer’s solution bathing the tissue was then replaced with 140 mM KGlu for 30 min, and the nuclei moving were counted again for 30 min. Although CBX had no effect on the basal movement of the microglia, it blocked the KGlu-induced movement (t test, n = 3 and P < 0.05).