FIG. 3.
Induction of IL-8 and repression of PTEN by hypoxia are RelA dependent. (A) U2OS cells were transfected with specific siRNA oligonucleotides prior to hypoxic stress exposure for the indicated times. IL-8 and PTEN mRNAs were analyzed by qPCR. (B) U2OS cells were treated and processed as for panel A, but qPCR was performed for RelA and p100 levels. (C) U2OS cells were transfected with specific siRNA oligonucleotides prior to hypoxic stress exposure for the indicated times. Whole-cell lysates were analyzed by Western blotting. (D) MCF10A cells were transfected with specific siRNA oligonucleotides prior to hypoxia exposure for the indicated times. IL-8 and PTEN mRNAs were analyzed by qPCR. *, P ≤ 0.050; **, P ≤ 0.010.