Fig. 2.
Analysis using Srebp2 minigene. (A) Schema of Srebp2 minigene used in this report. Arrows indicate RT primers. (B) RT-PCR analysis of 293T cells transfected with Srebp2 minigene. Note that there is no band around 2,000 bp, indicating that intron 16 was correctly spliced. (C) 293T cells were transfected with WT Abca1 3′UTR luciferase construct, along with expression plasmids. Values are means ± SE of four independent experiments. *P < 0.05 compared with empty vector (pcDNA3.1) or the Srebp2 minigene (exon 16–exon 17). (D) Western blot analysis of ABCA1 in THP-1 macrophages transfected with the Srebp2 minigene using lentivirus vectors. GAPDH was used as a loading control.