Fig. 3.
Effect of serum starvation in THP-1 macrophages. (A) Western blot analysis of ABCA1 protein levels under serum starvation conditions for the indicated time periods in THP-1 macrophages. GAPDH was used as a loading control. (B) Quantitative real-time PCR analysis of LDL receptor expression levels under serum starvation conditions for the indicated time periods in THP-1 macrophages. Values are mean ± SE of six independent experiments with normalization using GAPDH expression. *P < 0.05 compared with 10% FBS. (C) Quantitative real-time PCR analysis of SREBP2 expression levels under serum starvation conditions for the indicated time periods in THP-1 macrophages. Values are mean ± SE of six independent experiments with normalization using GAPDH expression. *P < 0.05 compared with 10% FBS. (D) Quantitative real-time PCR analysis of miR-33 under serum starvation conditions for the indicated time periods in THP-1 macrophages. Values are mean ± SE of six independent experiments with normalization using U6 snRNA. *P < 0.05 compared with 10% FBS.