Fig. 2.
Abca1-M/-M macrophages are hypersensitive to TLR2, 7, and 9, but not to TLR3 stimulation. A–C: Elicited PMs were treated with Pam3SCK4 (TLR2), Loxoribine (TLR7), or TLR9 for 0–12 h. The secretion of inflammatory factors was measured by ELISA. D, E: PMs were treated with poly (I:C) (TLR3) for 0–12 h. Cytokine or chemokine secretion was measured by ELISA. Results represent mean ± SEM of at least two independent experiments; n = 3 dishes of cells/group. * P<0.05, compared with WT. +/+: WT; -M/-M: Abca1-M/-M.