Fig. 3.
ABCA1 deficiency in macrophages resulted in increased membrane lipid raft content. Elicited PMs were incubated with or without MβCD for 0.5 h or MβCD-chol (80 µg/ml) for 1 h before staining with fPEG-chol. Cell fluorescence was analyzed using flow cytometry. A–C: Histograms show macrophage fPEG-chol staining in the absence (A) or presence (B) of MβCD or presence of MβCD-chol (C), respectively. D: Data from panels A–C are presented as mean ± SEM. Statistical significant differences between groups are indicated (*, P < 0.05; **, P < 0.01). Results are representative of two independent experiments with at least three mice per group. +/+: WT; -M/-M: Abca1-M/-M.