Fig. 5.
Increased TLR4 in lipid rafts of Abca1-M/-M macrophages. A and B: Lipid raft and nonraft membrane regions were isolated using a nondetergent method and analyzed for TLR4, clathrin (non-raft marker), and CD14 (raft marker) distribution by Western blotting. C: BMDMs were treated with 100 ng/ml LPS for 1 h and then lipid raft (fractions 1–8) and nonraft (fractions 10–13) fractions were pooled, and the distribution of the indicated proteins was analyzed by Western blotting. Results are representative of two independent experiments. D: TLR4 content of macrophage lipid rafts was calculated as a percentage of total membrane TLR4 (lipid rafts + nonrafts). Data are presented as mean ± range (n = 2). Statistically significant differences between genotypes are indicated (* P < 0.05). +/+: WT, -M/-M: Abca1-M/-M.