. 2010 Nov;51(11):3230–3242. doi: 10.1194/jlr.M007641

TABLE 1.

BA concentrations in livers of mice fed various BA-supplemented diets

BA (nmol/g liver)	Control		1% CA		0.3% DCA		0.3% CDCA		0.3% LCA		3% UDCA		2% Resin
BA (nmol/g liver)	M	F	M	F	M	F	M	F	M	F	M	F	M	F
TαMCA	6.9 ± 1.6	6.4 ± 1.0	2.0 ± 0.3^a	2.1 ± 0.5^a	2.8 ± 0.4^a	2.0 ± 0.3^a	31.8 ± 6.1^a	31.9 ± 4.5^a	20.0 ± 3.2^a	18.4 ± 3.2^a	2.5 ± 0.5^a	1.7 ± 0.3^a	2.4 ± 0.2^a	5.3 ± 1.0^b
αMCA	5.8 ± 0.8	3.0 ± 0.6^b	0.9 ± 0.1^a	0.3 ± 0.1^a ^b	0.5 ± 0.1^a	0.4 ± 0.0^a	30.5 ± 6.7^a	18.7 ± 2.4^a	7.6 ± 1.4	4.8 ± 0.8	16.6 ± 4.1	9.6 ± 2.6^a	2.1 ± 0.2^a	2.0 ± 0.2
TβMCA	34.2 ± 8.8	32.8 ± 7.3	2.7 ± 0.5^a	2.7 ± 0.3^a ^b	4.2 ± 0.9^a	5.3 ± 0.7^a	12.2 ± 2.3^a	19.0 ± 3.2	21.1 ± 7.8	24.2 ± 5.5	6.1 ± 1.1^a	6.9 ± 1.3^a	1.2 ± 0.2^a	3.0 ± 0.7^a ^b
βMCA	35.1 ± 5.0	21.9 ± 3.9	2.3 ± 0.4^a	0.8 ± 0.3^a	2.3 ± 0.8^a	2.7 ± 0.2^a	12.9 ± 1.8^a	14.4 ± 1.8	7.7 ± 2.7^a	7.0 ± 2.1^a	68.3 ± 18.3	66.0 ± 16.0^a	1.5 ± 0.3^a	1.4 ± 0.2^a
TωMCA	18.1 ± 4.5	11.3 ± 2.0	0.5 ± 0.1^a	0.5 ± 0.0^a	1.2 ± 0.3^a	1.6 ± 0.4^a	3.1 ± 0.3^a	3.6 ± 0.4^a	6.9 ± 2.9	8.8 ± 1.8	0.5 ± 0.1^a	0.5 ± 0.2^a	0.3 ± 0.1^a	0.6 ± 0.0^a ^b
ωMCA	11.8 ± 2.1	4.9 ± 0.8^b	0.4 ± 0.1^a	0.1 ± 0.0^a ^b	0.5 ± 0.1^a	0.5 ± 0.1^a	2.4 ± 0.4^a	1.7 ± 0.1^a	2.0 ± 0.9^a	1.8 ± 0.6^a	121.4 ± 39.7	95.6 ± 25.7^a	0.2 ± 0.0^a	0.2 ± 0.0^a
THCA	0.3 ± 0.1	0.3 ± 0.1	0.1 ± 0.0^a	0.1 ± 0.0^a	0.0 ± 0.0^a	0.1 ± 0.0^a	2.3 ± 0.1^a	1.9 ± 0.3^a	0.8 ± 0.2^a	1.0 ± 0.4^a	0.1 ± 0.0^a	0.0 ± 0.0^a	0.0 ± 0.0^a	0.1 ± 0.0^a ^b
HCA	0.1 ± 0.1	0.0 ± 0.0	0.1 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	1.0 ± 0.2	0.3 ± 0.1	0.0 ± 0.0	0.1 ± 0.1	0.4 ± 0.1	0.2 ± 0.0	0.0 ± 0.0	0.0 ± 0.0
TCA	86.1 ± 18.5	67.1 ± 11.5	298.5 ± 43.6^a	219.3 ± 32.3^a	122.5 ± 17.0	112.0 ± 21.8	6.0 ± 1.0^a	6.8 ± 0.8^a	29.1 ± 8.9^a	46.8 ± 10.1	1.8 ± 0.5^a	1.5 ± 0.4^a	49.4 ± 2.4	41.2 ± 4.6
GCA	0.1 ± 0.0	0.1 ± 0.1	0.5 ± 0.0^a	0.4 ± 0.1^a	0.2 ± 0.0	0.1 ± 0.0^b	0.0 ± 0.0^a	0.0 ± 0.0^a	0.0 ± 0.0	0.1 ± 0.0	0.0 ± 0.0^a	0.0 ± 0.0^a	0.1 ± 0.0	0.1 ± 0.0
CA	4.7 ± 0.9	2.7 ± 0.6	18.8 ± 1.3^a	3.6 ± 0.7^b	6.1 ± 1.0	3.8 ± 0.4	0.3 ± 0.1^a	0.2 ± 0.0^a	0.8 ± 0.3^a	0.7 ± 0.2^a	0.9 ± 0.3^a	0.7 ± 0.1^a	3.1 ± 0.6	1.2 ± 0.2^a ^b
TDCA	7.7 ± 1.6	7.0 ± 1.3	38.4 ± 3.3^a	36.7 ± 5.0^a	57.2 ± 7.9^a	31.8 ± 4.7^a ^b	2.1 ± 0.2^a	1.8 ± 0.1^a	7.6 ± 1.3	11.3 ± 4.2	0.9 ± 0.3^a	0.8 ± 0.3^a	7.3 ± 1.2	7.3 ± 0.4
GDCA	0.0 ± 0.0	0.0 ± 0.0	0.1 ± 0.0^a	0.1 ± 0.0^a	0.2 ± 0.0^a	0.1 ± 0.0^a ^b	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0^a	0.1 ± 0.1	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0
DCA	0.4 ± 0.1	0.4 ± 0.1	5.2 ± 0.4^a	1.8 ± 0.4^a ^b	5.1 ± 1.5^a	1.3 ± 0.1^a ^b	0.2 ± 0.0	0.1 ± 0.0^a ^b	0.3 ± 0.0	0.3 ± 0.0	0.8 ± 0.2	0.6 ± 0.1	0.4 ± 0.1	0.3 ± 0.0
isoDCA	0.0 ± 0.0	0.0 ± 0.0	0.3 ± 0.0^a	0.2 ± 0.1^a ^b	0.5 ± 0.2^a	0.1 ± 0.0^a ^b	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.4 ± 0.0	0.0 ± 0.0	0.0 ± 0.0
7-oxoDCA	5.1 ± 1.0	1.7 ± 0.5^b	1.2 ± 0.3^a	0.1 ± 0.0^a ^b	1.0 ± 0.2^a	0.4 ± 0.0^a ^b	9.3 ± 1.6^a	3.7 ± 0.7^a ^b	1.8 ± 0.5^a	0.6 ± 0.1^a ^b	0.4 ± 0.1^a	0.1 ± 0.0^a	1.2 ± 0.4^a	0.4 ± 0.1^a ^b
TCDCA	3.0 ± 0.4	3.2 ± 0.3	1.6 ± 0.2^a	1.4 ± 0.2^a	1.4 ± 0.1^a	1.3 ± 0.2^a	43.1 ± 8.8^a	33.9 ± 6.8^a	22.7 ± 5.1^a	15.9 ± 3.8^a	7.9 ± 1.8^a	6.7 ± 1.4^a	4.0 ± 0.3	8.1 ± 1.3^a ^b
GCDCA	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.2 ± 0.0^a	0.1 ± 0.1^a	0.0 ± 0.0	0.1 ± 0.1	0.3 ± 0.1^a	0.2 ± 0.0^a	0.0 ± 0.0	0.0 ± 0.0
CDCA	1.6 ± 0.1	0.9 ± 0.1	0.5 ± 0.1^a	0.2 ± 0.0^a ^b	0.2 ± 0.0^a	0.2 ± 0.0^a	36.0 ± 10.1^a	14.1 ± 3.3^a ^b	6.6 ± 2.0^a	6.7 ± 4.2^a	19.6 ± 7.6^a	13.2 ± 2.3^a	2.0 ± 0.2	2.1 ± 0.3^a
12-oxoCDCA	12.1 ± 1.8	7.4 ± 1.9	1.0 ± 0.3^a	0.3 ± 0.1^a ^b	1.1 ± 0.4^a	0.7 ± 0.1^a ^b	6.4 ± 1.4^a	3.2 ± 0.5^a ^b	3.7 ± 1.0^a	2.8 ± 0.5^a	4.8 ± 1.2	3.1 ± 1.3	0.5 ± 0.2^a	0.3 ± 0.0^a
TUDCA	3.0 ± 0.5	3.3 ± 0.6	0.9 ± 0.1^a	0.8 ± 0.2^a	0.6 ± 0.1^a	0.9 ± 0.1^a ^b	12.4 ± 1.6^a	13.0 ± 2.2^a	5.6 ± 0.8^a	5.1 ± 0.8	202.9 ± 21.4^a	223.3 ± 32.7^a	0.4 ± 0.1^a	0.8 ± 0.1^a ^b
GUDCA	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	16.5 ± 3.3^a	22.2 ± 3.7^a	ND	ND
UDCA	0.9 ± 0.1	0.8 ± 0.2	0.3 ± 0.1^a	0.1 ± 0.0^a ^b	0.1 ± 0.0^a	0.5 ± 0.3^b	6.7 ± 1.3^a	4.8 ± 0.8^a	0.9 ± 0.2	1.2 ± 0.3	875.4 ± 198.0^a	1038 ± 197.3^a	0.2 ± 0.0^a	0.4 ± 0.3
TMDCA	1.2 ± 0.4	1.4 ± 0.4	0.2 ± 0.0^a	0.5 ± 0.2^b	0.6 ± 0.1	0.4 ± 0.1^a	4.1 ± 0.6^a	5.0 ± 0.9^a	38.8 ± 10.7^a	17.1 ± 4.5^a	7.0 ± 2.2^a	6.9 ± 2.6^a	0.3 ± 0.1	1.2 ± 0.2^b
MDCA	1.1 ± 0.2	0.8 ± 0.2	0.2 ± 0.0^a	0.1 ± 0.0^a ^b	0.3 ± 0.0^a	0.2 ± 0.0^a	4.2 ± 0.5^a	3.3 ± 0.5^a	22.5 ± 5.3^a	13.4 ± 5.4^a	32.5 ± 9.9^a	23.5 ± 7.1^a	0.6 ± 0.1	0.8 ± 0.1
THDCA	1.7 ± 0.5	1.4 ± 0.3	0.1 ± 0.0^a	0.2 ± 0.0^a	0.6 ± 0.2	0.4 ± 0.1^a	2.3 ± 0.4	2.2 ± 0.4	10.6 ± 2.5^a	7.0 ± 1.9^a			0.1 ± 0.0^a	0.3 ± 0.1^a ^b
HDCA	1.1 ± 0.3	0.5 ± 0.2	0.1 ± 0.0^a	0.0 ± 0.0^a ^b	0.2 ± 0.0^a	0.1 ± 0.0^a	1.8 ± 0.2	1.1 ± 0.2^a	6.0 ± 2.1^a	4.2 ± 1.7^a	2.6 ± 1.1	2.1 ± 1.7	0.1 ± 0.0^a	0.1 ± 0.0^a ^b
TLCA	0.6 ± 0.1	0.4 ± 0.0	0.2 ± 0.0^a	0.2 ± 0.0^a	0.3 ± 0.0^a	0.3 ± 0.0^a	6.2 ± 0.7^a	4.5 ± 0.6^a	25.0 ± 6.2^a	9.6 ± 2.3^a	11.1 ± 3.8^a	16.5 ± 10.4^a	0.9 ± 0.1	2.6 ± 0.3^a ^b
GLCA	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	0.6 ± 0.3^a	0.4 ± 0.1^a	ND	ND
LCA	0.1 ± 0.0	0.0 ± 0.0	0.1 ± 0.0^a	0.0 ± 0.0	0.0 ± 0.0^a	0.0 ± 0.0	1.1 ± 0.2^a	0.4 ± 0.0^a ^b	3.1 ± 0.9^a	2.6 ± 1.8^a	47.5 ± 18.0^a	43.5 ± 17.8^a	0.1 ± 0.0	0.2 ± 0.0^a ^b
6-oxoLCA	0.1 ± 0.0	0.0 ± 0.0	0.0 ± 0.0^a	0.0 ± 0.0	0.0 ± 0.0^a	0.0 ± 0.0	0.2 ± 0.0^a	0.1 ± 0.0^a ^b	0.7 ± 0.2^a	0.2 ± 0.1^a ^b	0.4 ± 0.1	0.1 ± 0.1	0.0 ± 0.0	0.0 ± 0.0
7-oxoLCA	0.1 ± 0.0	0.0 ± 0.0^b	0.0 ± 0.0^a	0.0 ± 0.0	0.0 ± 0.0^a	0.0 ± 0.0	1.3 ± 0.4^a	0.2 ± 0.0^a ^b	0.2 ± 0.0^a	0.0 ± 0.0^a	2.4 ± 0.8^a	0.6 ± 0.1^a	0.1 ± 0.0	0.0 ± 0.0^b
12-oxoLCA	0.3 ± 0.1	0.1 ± 0.1^b	1.2 ± 0.3^a	0.2 ± 0.1^b	1.0 ± 0.3^a	0.1 ± 0.0^b	0.1 ± 0.0^a	0.0 ± 0.0^b	0.2 ± 0.1	0.0 ± 0.0^b	0.0 ± 0.0^a	0.0 ± 0.0^a	0.2 ± 0.1	0.0 ± 0.0
dehydroLCA	ND	ND	ND	ND	ND	ND	ND	ND	0.1 ± 0.0^a	0.0 ± 0.0^a	1.3 ± 0.5^a	0.5 ± 0.2^a	ND	ND
isoLCA	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.1 ± 0.0^a	0.0 ± 0.0	0.2 ± 0.1^a	0.4 ± 0.4^a	2.5 ± 1.0^a	1.3 ± 0.8^a	0.0 ± 0.0	0.0 ± 0.0

Values are expressed as mean ± SEM for five mice in each group. BA, bile acid; BAG, bile acid glucuronide; CA, cholic acid; CDCA, chenodeoxycholic acid; DCA, deoxycholic acid; F, female; GCA, glycocholic acid; GCDCA, glycochenodeoxycholic acid; GDCA, glycodeoxycholic acid; GLCA, glycolithocholic acid; GUDCA, glycoursodeoxycholic acid; HCA, hyocholic acid; HDCA, hyodeoxycholic acid; isoLCA, iso-lithocholic acid; LCA, lithocholic acid; 7-oxoDCA, 3α,12α-dihydroxy-7-oxo-5β–cholan-24-oic acid; 6-oxoLCA, 3α-hydroxy-6-oxo-5β–cholan-24-oic acid; 7-oxoLCA, 3α-hydroxy-7-oxo-5β–cholan-24-oic acid; 12-oxoLCA, 3α-hydroxy-12-oxo-5β–cholan-24-oic acid; M, male; ωMCA, ω-muricholic acid; MDCA, murideoxycholic acid; ND, not detectable; TCA, taurocholic acid; TCDCA, taurochenodeoxycholic acid; THCA, taurohyocholic acid; TLCA, taurolithocholic acid; TαMCA, tauro-α-muricholic acid; TβMCA, tauro-β-muricholic acid; TωMCA, tauro-ω-muricholic acid; TMDCA, tauromurideoxycholic acid; TUDCA, tauroursodeoxycholic acid; UDCA, ursodeoxycholic acid.

Significant difference between the same gender of control and BA-fed groups (P < 0.05).

Significant difference between male and female mouse livers in the same group (P < 0.05).