Table II. Quantitative analysis of proteins increased in lung membrane rafts of caveolin-1 KO versus WT mice.
-Fold change reflects data from three independent experiments. Each protein was identified independently both by Paragon and Mascot database search engines using the IPI mouse database. False discovery rates were determined by running PeptideProphet and ProteinProphet analyses on Mascot results. Raw data and data results are available at http://yped.med.yale.edu/repository (access code, aSKezE).
| Protein ID | Protein name | No. of distinct peptides in ratios | ProteinProphet score | ProteinPilot protein score | -Fold change | Grp |
|---|---|---|---|---|---|---|
| IPI00323230 | Spectrin a chain | 6 | 1 | 218.04 | 1.214358 | 1.14e−08 |
| IPI00307966 | ADP-ribosyl cyclase 1 | 11 | 1 | 17.07 | 1.215031 | 1.63e−07 |
| IPI00117735 | Myelin P0 protein precursor | 6 | 1 | 6.48 | 1.221400 | 0.000288 |
| IPI00679092 | Similar to spectrin a 1 | 5 | 1 | 8.36 | 1.225561 | 1.75e−08 |
| IPI00131176 | Cytochrome c oxidase subunit 2 | 4 | 1 | 8.56 | 1.243182 | 0.003629 |
| IPI00109727 | Thy-1 membrane glycoprotein precursor | 3 | 1 | 6 | 1.261946 | 0.000248 |
| IPI00123746 | Cadherin-13 precursor | 10 | 1 | 19.23 | 1.317579 | 0.000150 |
| IPI00223047 | Cytoskeleton-associated protein 4 | 13 | 1 | 20.8 | 1.335605 | 2.63e−06 |
| IPI00120719 | Cytochrome c oxidase, subunit Va | 20 | 1 | 32.69 | 1.38002 | 1.03e−05 |
| IPI00131695 | Serum albumin precursor | 27 | 1 | 51.18 | 1.385615 | 9.60e−09 |
| IPI00121550 | Na+/K+-transporting ATPase subunit β-1 | 5 | 1 | 10.26 | 1.39774 | 0.001573 |
| IPI00116154 | Cytochrome c oxidase, subunit Vb | 7 | 1 | 14 | 1.42061 | 1.64e−07 |
| IPI00128642 | Lung carbonyl reductase | 5 | 1 | 12.19 | 1.446933 | 9.37e−06 |
| IPI00133956 | Gpihbp1 | 2 | 1 | 3.1 | 1.453672 | 2.84e−05 |
| IPI00225390 | Cytochrome c oxidase subunit VIb isoform 1 | 8 | 1 | 16.44 | 1.460724 | 6.27e−07 |
| IPI00170093 | Ndufs8 | 5 | 1 | 5.73 | 1.489794 | 2.96e−05 |
| IPI00121209 | Apolipoprotein A-I precursor | 25 | 1 | 26.07 | 1.407229 | 2.56e−05 |
| IPI00121319 | Cysteine-rich protein 2 | 4 | 0.94 | 5.55 | 1.513123 | 0.000772 |
| IPI00133562 | Chchd3 | 10 | 1 | 16.1 | 1.533374 | 3.45e−07 |
| IPI00230540 | VDAC1 | 7 | 1 | 15.4 | 1.544335 | 2.38e−08 |
| IPI00133006 | Acyl carrier protein | 6 | 1 | 10.28 | 1.562844 | 2.72e−05 |
| IPI00121288 | Ndufb10 | 2 | 0.97 | 4.47 | 1.641092 | 2.13e−05 |
| IPI00126208 | Hemoglobin, β adult major chain | 3 | 1 | 14.03 | 1.66103 | 8.25e−09 |
| IPI00315480 | Surfactant-associated protein A | 13 | 1 | 20.97 | 1.661763 | 4.56e−05 |
| IPI00132474 | Itgb1 | 8 | 1 | 14.96 | 1.663839 | 5.88e−07 |
| IPI00311682 | Atp1a1 | 13 | 1 | 22.67 | 1.727181 | 1.71e−05 |
| IPI00229008 | Ndufs4 | 6 | 1 | 11.07 | 1.81813 | 8.10e−09 |
| IPI00115522 | Fibrinogen, a polypeptide | 3 | 0.94 | 5.53 | 1.915084 | 4.08e−06 |
| IPI00119618 | Calnexin precursor | 10 | 1 | 20.67 | 2.138918 | 0.000461 |
| IPI00316491 | Hemoglobin β-2 subunit | 6 | 1 | 29.92 | 2.096561 | 3.92e−11 |
| IPI00110658 | Hemoglobin, β adult major chain | 3 | 1 | 18.65 | 2.17418 | 5.65e−09 |
| IPI00230113 | Cytochrome b5 | 6 | 1 | 10.33 | 2.204051 | 1.93e−11 |
| IPI00469114 | Hemoglobin a subunit | 3 | 1 | 2.36 | 2.230882 | 8.95e−09 |
| IPI00387318 | Tmem30a | 3 | 1 | 5.15 | 3.114185 | 5.10e−08 |
| IPI00623553 | ATP synthase D chain, mitochondrial | 10 | 1 | 16.4 | 3.389328 | 7.95e−10 |
| IPI00555000 | Uqcrb | 8 | 1 | 10.79 | 4.012617 | 1.58e−09 |