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. 2010 Oct 19;1(4):e00219-10. doi: 10.1128/mBio.00219-10

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Copyright © 2010 Zhou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 3 — MHV-induced upregulation of IFN-β, MDA5, and RIG-I in BMM is dependent on IFN-α/β receptor (IFNAR). BMM isolated from WT, IFNAR^−/−, or MyD88^−/− mice were infected with MHV (A and C) or SenV (E) or transfected with poly(I ⋅ C) (D). (A) MHV titers were determined in cell supernatants at 16 hours postinfection as described in Materials and Methods. (B) mRNA was harvested from mock-infected BMM, and basal levels of IFN-β, MDA5, and RIG-I were measured by real-time qPCR. (C to E) mRNA was harvested 16 h after infection or transfection. mRNA levels were measured by real-time qPCR. C_T ratios to HPRT are shown. MHV induced upregulation to a greater extent than poly(I ⋅ C) or SenV did (note the differences in scale in panels B to E). Two or three replicates were performed in each experiment, and one of three independent experiments is shown. Values for IFNAR^−/− or MyD88^−/− BMM that were statistically significantly different from the values for wild-type cells are indicated as follows: *, P < 0.05; **, P < 0.01.