Table 1. Intensities of the RNA–mutant probe complexes expressed as percentages relative to TNF-10–protein complexes.

Experiments were performed with equal amounts of each of the cRNA probes. In order to further ensure that equal amount of probes were used in the reactions, data were normalized by determining the quantity of cRNA free probe present in the control lanes (without protein extract) of both the non-mutant (TNF-10) and the mutant (TNF-10m, TNF-10m2) probes. Quantification of the intensities of the complexes were then adjusted by taking into account differences in the free probes.

Results are representative of four independent experiments.