Table 2. Activities of the mitochondrial respiratory chain complexes in the muscle and myocardium.
| Sample | ||||||
|---|---|---|---|---|---|---|
| Muscle mitochondria | Myocardium homogenate | |||||
| Assay | Control muscle mitochondria n=50 | B3 | A5 | Control myocardium n=3 | A8 | A5 |
| Citrate synthasea | 1990±370 | 1690 | 1800 | 251±164 | 454 | 233 |
| NADH-CoQ reductasea (complex I) | 241±92 | 149 (74%) | 110 (50%) | |||
| NADH-cytochrome c reductasea (complex I+III) | 586±234 | 413 (84%) | 335 (73%) | |||
| Succinate – cytochrome c reductasea (complex II+III) | 312±118 | 133 (51%) | 115 (64%) | 27±13 | 10 (21%) | 2 (8%) |
| Succinate-CoQ reductasea (complex II) | 72±26 | 30 (50%) | 39 (60%) | |||
| Succinate dehydrogenasea (complex II) | 260±86 | 118 (54%) | 131 (56%) | 48±25 | 13 (15%) | 8 (18%) |
| Ubiquinol-cytochrome c reductasea (complex III) | 4270±970 | 3800 (106%) | 3000 (99%) | |||
| Cytochrome c oxidasea (complex IV) | 1155±420 | 1135 (117%) | 1128 (108%) | 197±118 | 354 (100%) | 147 (81%) |
| Mg++ATPase (complex V)a | 536±193 | 828 (184%) | 675 (111%) | |||
| Pyruvate+malate oxidationb | 67±32 | 59 (105%) | ||||
| Succinate oxidationb | 94±36 | 20 (26%) | ||||
The assignment of patients is according to Figure 1.
a
Activities of the five mitochondrial respiratory chain complexes were measured spectrophotometrically in nmol/min per mg.
b
Activities of the five mitochondrial respiratory chain complexes were measured by oxygen consumption in nat O/min per mg protein.
Values in brackets present activities as a percentage of the control mean, normalized for citrate synthase activity.