FIG. 6.
The H2O2-mediated increase in Nox2 is reduced by ebselen in the diabetic ApoE−/−GPx1−/− (dKO) kidney. A: Quantitative RT-PCR analysis of Nox2 in ApoE−/− and dKO kidneys after 20 weeks of diabetes; n = 8–10 kidneys/group. B: Representative photomicrographs of kidney glomeruli (i–iv) and tubules (v–viii); nondiabetic dKO (i and v); diabetic dKO (ii and vi); diabetic dKO+Eb (iii and vii); and negative control which consisted of species-matched nonimmune IgG in place of primary antibody (iv and viii). C and D: Quantitation of Nox2 protein within glomeruli and tubules, respectively. ***P < 0.001; **P < 0.01; *P < 0.05 diabetic dKO vs. diabetic ApoE−/− kidneys; and versus nondiabetic counterparts. ##P < 0.01 and ###P < 0.001 vs. diabetic dKO kidneys. Bars, mean ± SEM; n = 20 glomeruli/kidney and 4–6 kidneys/group and n = 6 tubular fields/kidney and at least 5 kidneys/group. a.u., arbitrary units; D, diabetic; Eb, ebselen; ND, nondiabetic. (A high-quality digital representation of this figure is available in the online issue.)