. 2003 Jun;9(6):623–627. doi: 10.3201/eid0906.020377

Table 1. Heat inactivation of B. anthracis spores^a.

Temperature	Time	Inoculum size	Inactivation effect	Ref.
Boiling
100°C	10 min	3 x 10⁶	Sample sterilized	4,5
100°C	5 min	7.5 x 10⁸	Sample sterilized	4,5
Moist heat
90°C	20 min	1.2 x 10⁶	Sample sterilized	4,5
90°C to 91°C	60 min	3 x 10⁸	Spores detected	4,5
100°C	10 min	1.2 x 10⁶	Sample sterilized	5,6
100°C to 101°C	17 min	1 x 10⁵	Sample sterilized	5,6
105°C	10 min	3 x 10⁶	Sample sterilized	5
120°C	15 min	2.4 x 10⁸	Sample sterilized	4
Dry heat
140°C	>90 min	6 x 10³ to 1.2 x 10⁴	Sample sterilized	7
150°C	10 min	6 x 10³ to 1.2 x 10⁴	Sample sterilized
160°C	10 min	6 x 10³ to 1.2 x 10⁴	Sample sterilized
180°C	2 min	6 x 10³ to 1.2 x 10⁴	Sample sterilized
190°C	1 min	6 x 10³ to 1.2 x 10⁴	Sample sterilized
200°C	30 sec	6 x 10³ to 1.2 x 10⁴	Sample sterilized

^aSpores in liquid suspension exposed to flowing steam at 100°C.

Table 1. Heat inactivation of B. anthracis sporesa.