Figure 4. Brown adipose tissue (BAT) function test.
Change of BAT temperature in response to a β3-agonist, CL316243, was examined in chloralose- (75 mg kg−1, i.p.) and urethane- (750 mg kg−1, i.p.) anaesthetized mice. Orexin knockout mice (ORX-KO), orexin neuron-ablated mice (ORX-AB) and their corresponding wild-type litter mates (WTKO and WTAB) received i.p. injection of saline (dashed line) or CL316243 (1 mg kg−1, continuous line). Data are presented as mean ±s.e.m. of the peak values during the observation period of 120 min (n = 5 for each group). *P < 0.05 vs. pre-treatment value.