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. Author manuscript; available in PMC: 2011 Nov 23.

Published in final edited form as: Circulation. 2010 Nov 8;122(21):2170–2182. doi: 10.1161/CIRCULATIONAHA.110.958033

Heart homogenates were prepared from the ischemic area in Tg-Sirt1 and NTg mice subjected to I/R. Expression of Bax, MnSOD, Trx1, Bcl-xL, cleaved caspase-3 and tubulin was evaluated by immunoblots. (A) Representative immunoblots are shown. (B-F) The quantitative and statistical analyses of the immunoblots are shown. The relative expression of MnSOD (B), Trx1 (C), Bcl-xL (D), Bax (E) and cleaved caspase-3 (F) in Tg-Sirt1 versus NTg is shown. The expression level of NTg mice is expressed as 1. (G-I) Representative immunostaining of Bcl-xL (G), cleaved caspase-3 (H), and 8-OHdG (I) in the ischemic area of left ventricular myocardial sections in Tg-Sirt1 and NTg mice subjected to I/R.

Heart homogenates were prepared from the ischemic area in Tg-Sirt1 and NTg mice subjected to I/R. Expression of Bax, MnSOD, Trx1, Bcl-xL, cleaved caspase-3 and tubulin was evaluated by immunoblots. (A) Representative immunoblots are shown. (B-F) The quantitative and statistical analyses of the immunoblots are shown. The relative expression of MnSOD (B), Trx1 (C), Bcl-xL (D), Bax (E) and cleaved caspase-3 (F) in Tg-Sirt1 versus NTg is shown. The expression level of NTg mice is expressed as 1. (G-I) Representative immunostaining of Bcl-xL (G), cleaved caspase-3 (H), and 8-OHdG (I) in the ischemic area of left ventricular myocardial sections in Tg-Sirt1 and NTg mice subjected to I/R.