Figure 5. Continuity of transcript coverage.
(a) Average number of segments (separated by at least five bases of zero coverage) weighted by the average expression of each gene, in each library. Full data for all library variations are presented in Supplementary Table 2. (b–e) Fraction of bases not covered by reads for each gene (blue dot) in the genome, plotted against the fraction of total reads for that gene in the pooled library, for the dUTP method (c), the 3’ split adaptor method (d) and the SMART method (e). In each case, a Lowess fit is shown as a red curve, with fits from all libraries shown in (b). Full data for all library variations are presented in Supplementary Fig. 2.