Table 3.
DTNB Reductase Activity of Truncated TR after H2O2 Incubation.a
H2O2 (mM) | Activity (mol NADPH/min/mol of enzyme) |
---|---|
0 | 640 ± 80 |
0.5 | 600 ± 40 |
1 | 580 ± 35 |
10 | 530 ± 10 |
50 | 670 ± 40 |
The concentration of DTNB in this assay was constant at 0.5 mM. For other assay conditions see text.