Fig. 5. Apicoplast morphology throughout the asexual life cycle of blood stage parasites expressing targeted GFP. Stages represent ring form (A), small trophozoite (B), large trophozoite (C), three stages of early schizonts (D–F), late schizont (G) and free merozoites (H). Single apicoplasts enlarge slowly, elongate to branched forms during schizogony and then divide and segregate prior to merozoite egression. These stages were seen for all transfectants with apicoplast-targeted GFP. (A–E) and (G–H) represent ACPleader–GFP-expressing parasites and (F) represents a FabHleader[T40/49A]–GFP-expressing parasite. Scale bars, 5 µm.