Fig. 2. Effects of Nek2B inhibition on the meiotic Cdc2 cycle. (A) Effect on the initiation of oocyte maturation. Thirty immature oocytes were uninjected (None) or injected with Nek2B-ΔC mRNA (Nek2B-ΔC; 10 ng/oocyte), Nek2B-KR mRNA (Nek2B-KR;10 ng/oocyte), preimmune antibody (IgG; 300 ng/oocyte) or anti-Nek2B antibody (α-Nek2B; 300 ng/oocyte), and 2 h later were treated with progesterone and scored for the percentage GVBD. (B) Effect on the meiotic Cdc2 cycle. Oocytes treated as in (A) were collected at the times indicated and subjected to H1 kinase assays of Cdc2. H1 kinase activity is represented in arbitrary units. For both (A) and (B), similar results were obtained in two other independent experiments.