Fig. 3. Effect of Nek2B inhibition on the mitotic Cdc2 cycle. Thirty unfertilized eggs were injected with Nek2B-ΔC mRNA (Nek2B-ΔC; 10 ng/egg), Nek2B-KR mRNA (Nek2B-KR; 10 ng/egg), preimmune antibody (IgG; 300 ng/egg) or anti-Nek2B antibody (α-Nek2B;300 ng/egg); in these eggs, the action of injection itself caused egg activation. Activated eggs were collected at the times indicated and subjected to H1 kinase assays as in Figure 2B. Results similar to those presented here were obtained in two other independent experiments.