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Fig. 7. Frameshifting of C.elegans antizyme (AZ) mRNA in reticulocyte lysates. Translation products were separated on a 17.5% SDS–PAGE gel. Normally in vitro translation reactions contain 0.5 mM spermidine (‘–’, 0.5 mM and ‘+’, 1.0 mM spermidine). IFC, in-frame control.