. Author manuscript; available in PMC: 2011 Jan 28.

Published in final edited form as: Semin Immunol. 2005 Oct;17(5):370–377. doi: 10.1016/j.smim.2005.05.007

Table 1.

Responses following anti-CD4 treatment of young and aged mice

Mice^a	Treatment	T cell proliferation^b	IL-2 production^c	Helper function^d
Young	Anti-CD4	++++	++++	++++
Young	Isotype	++++	++++	++++
Aged	Anti-CD4	++++	++++	++++
Aged	Isotype	++	++	++

Young and aged TCR Tg mice were treated with anti-CD4 or isotype control antibody. After 8 weeks, CD4 cells were purified from spleen and LNs and stimulated in vitro with peptide Ag and APC.

T cell proliferation was determined by the total number of blasts recovered after 4 days of culture with Ag/APC.

IL-2 production after 24 h of culture with Ag/APC was determined by collecting supernatants and assaying for IL-2 in a bioassay.

Young and aged B10.BR mice were treated with anti-CD4 or isotype control antibody. After 8 weeks, mice were immunized with NP-PCC/alum. The number of responding NP-specific B cells was determined by flow cytometry.