FIG. 8.
BgaB reporter fusions. (A) Schematic representation of the different clpX promoter fragments fused to the bgaB reporter gene. The terminator of the preceding tig gene is shown at the left. The putative −35 and −10 boxes of the promoters, the Shine-Dalgarno (SD) sequence of the spoVG gene, and the translational start codon (ATG) of bgaB are indicated. (Diagram 1) DNA fragment carrying both putative σA- and σB-dependent promoters in the wild-type background (BUG9), the ctsR mutant background (BUG10), the sigB mutant background (BUG11), or the clpX mutant background (BUG12). (Diagram 2) DNA fragment carrying only the putative σB-dependent promoter in the wild-type background (BUG13) or the sigB mutant background (BUG14). (B) Transcription of clpX measured as BgaB activity in Miller units.