Fig. 3.
Phagocytosed Bb colocalizes with TLR2 and TLR8 in human monocytes. A composite of representative confocal micrographs shows three consecutive Z-stack planes of GFP-Bb–stimulated human monocytes. Extracellular Bb interacts with TLR2 at the plasma membrane of the monocyte (A), whereas recently internalized (bacterial coil) and fully degraded (fluorescent bleb) Bb colocalizes with TLR2 (B) and TLR8 (C). Mander's colocalization coefficient values (M2), shown in the upper right corner of each figure, are indicative of the proportion of the green signal overlapping with the signal in the red channel. Red depicts TLR2 signals in A and B and TLR8 in C, green depicts GFP-Bb, and white represents red and green pixels that colocalize. (Scale bar, 5 μm.) Colocalization through the Z-stack images illustrated in A and B (TLR2) and in C (TLR8) is shown in Movie S1, Movie S2, and Movie S3.