Figure 3.
IL-2 and PDK1 but Not Akt Sustain Glucose Uptake in CTL
(A) 2-deoxyglucose (2DOG) uptake by wild-type splenic T cells activated with 2C11 for 48 hr and then cultured in IL-2 for 5 days, with some cells being deprived of IL-2 for the last 24 hr of culture.
(B) Wild-type splenocytes were activated with 2C11 and IL-2 for 2 days and then cultured in IL-2 in standard media for 72 hr. For the final 48 hr of culture, cells were placed in media containing indicated glucose concentrations and the fold change in cell number assessed.
(C) Relative 2DOG uptake in wild-type splenic T cells activated with 2C11 for 48 hr then cultured in IL-2 for an additional 5 days, with 1 μM AktI being added for the last 48 hr of culture.
(D and E) Relative 2DOG uptake (D) and phenylalanine uptake (E) by Cre control (Pdpk1+/+/ tm9(creEsr1)Arte +4OHT) and Pdpk1-null (Pdpk1fl/fl/ tm9(creEsr1)Arte +4OHT) CTL.
(F) Naive P14 T cells were maintained in IL-7 or stimulated for 18 hr with gp33 in the presence or absence of AktI or IC87114 and then their ability to take up 2DOG was assayed.
Data are representative of 3 (B, D, F), 5 (A), or 11 (C) experiments; mean ± SEM of biological replicates, except (F), representative data from 2 experiments; mean ± SD of technical replicates.