Table 1.
Antibodies
| Primary antibodies | Species | Catalogue no. | Dilution | Source |
|---|---|---|---|---|
| PDGFr α (for acetone fixtative) | Rat | 16–1401 | 1:500 | eBioscience, CA, USA |
| PDGFr α (for 4% PFA fixtative) | Goat | AF1062 | 1:200 | R & D Systems, MN, USA |
| c-Kit | Goat | AF1356 | 1:500 | R & D Systems, MN, USA |
| SK3 | Rabbit | APC-025 | 1:500 | Alomone Labs, Israel |
| PGP 9.5 | Rabbit | RA-95101 | 1:500 | UltraClone Ltd, UK |
| nNOS | Sheep | 1:200 | Gift from Dr Piers Emson, Mol. Sci. Group, Cambridge, UK | |
| vAChT | Goat | ab34852 | 1:200 | Abcam, MA, USA |
| Secondary antibodies | Catalogue no. | Dilution | Source | |
| Alexa594 labelled donkey anti-rabbit IgG | A-21207 | 1:500 | Molecular Probes/Invitrogen | |
| Alexa488 labelled donkey anti-rat IgG | A-21208 | 1:500 | Molecular Probes/Invitrogen | |
| Alexa488 labelled donkey anti-goat IgG | A-11055 | 1:500 | Molecular Probes/Invitrogen | |
| Alexa594 labelled donkey anti-goat IgG | A-11058 | 1:500 | Molecular Probes/Invitrogen | |
| Alexa594 labelled donkey anti-sheep IgG | A-11016 | 1:500 | Molecular Probes/Invitrogen | |