Figure 3.
Decrease of LPS-induced inflammatory factor mRNA expression in PVN and SFO by angiotensin II AT1 receptor blockade. Short-term candesartan treatment was followed by LPS injection as described in Materials and Methods. Expression of mRNAs of proinflammatory factors and mediators was detected in microdissected PVN and SFO by real-time PCR and was normalized to the level of GAPDH mRNA. Results are expressed as a fold change relative to vehicle-saline group. Data represent mean±SEM (n=5–8). *P<0.05, **P<0.01, ***P<0.001, compared with vehicle-saline group; #P<0.05, ##P<0.01, ##P<0.001, compared with vehicle-LPS group.