Fig. 4. Analysis of U24 processing in different mutant strains. After 1 h shift at the non-permissive temperature (time 0), cultures were induced with galactose for the indicated times. Northern blot analysis of RNA was performed with the CH1462 strain (WT) or the strains carrying the mutant alleles rna14-1, pap1-5, rnt1Δ, rat1-1 and nup145N transformed with GAL/U24* (A) or GAL/U24*S (B) plasmids. Both blots were hybridized with a 32P-labeled tag-specific oligonucleotide. Size markers (MspI-digested pBR322) and schematic representations of the different processing products are indicated at the sides. The lower panels show control hybridizations with an snR13 snoRNA-specific probe.