Figure 2. CpG-motif and CG-rich containing DNA fragments activate TLR9.
The canonical CpG motif recognized by TLR9, PuPuCGPyPy is depicted in turquoise. The strong B cell mitogen 1826, a single stranded phosphorothioate (PS-DNA) oligonucleotide (ODN), contains two repeats of the CpG motif. CG50 is an experimentally designed phosphorodiester dsDNA fragment that includes 50 repeats of the canonical CpG motif. Clone 11 is a dsDNA fragment corresponding to an endogenous CpG island that contains two CpG motifs (in turquoise) and 42 CG-repeats (in yellow). SenP1 is a DNA fragment representative of genomic DNA.