FIG. 3.
Effect of 8-Br-cAMP on thrombin-induced lymphatic endothelial barrier dysfunction. (A) Time-course of thrombin-induced changes in mean HMLEC-d TER in cells pretreated with 8-Br-cAMP or vehicle (EBM). (B) 8-Br-cAMP caused an increase in TER but in this experiment the maximal change elicited by 8-Br-cAMP was not significantly different from vehicle (P = 0.09). (C) Pretreatment of HMLEC-d with 8-Br-cAMP (8-Br) did not significantly affect that maximum decrease in TER elicited by thrombin (T) when compared to the time point just prior to the thrombin addition. (D) The overall change in TER compared to time = 0 min (just before addition of 8-Br-cAMP) was significantly attenuated in cells treated with both 8-Br-cAMP and thrombin compared to thrombin alone. N = 8 for both groups.