Table II. Refolding time constants determined by reverse-phase HPLC for the disappearance of the fully reduced protein (R), the formation (τ1) and disappearance (τ2) of des-[76-94] and the formation of the native state (N) during refolding of fully reduced lysozyme. In addition the time constants obtained for the conversion of purified des-[76-94] to the native state are shown.
| Protein species | τ1 (min) |
τ2 (min) |
||
|---|---|---|---|---|
| –Ficoll | +Ficoll | –Ficoll | +Ficoll | |
| Ra | 1.45 ± 0.04 | 1.09 ± 0.15 | – | – |
| des-[76-94]a | 16.8 ± 1.9 | 9.9 ± 1.6 | 209 ± 36 | 278 ± 45 |
| Na | 20.7 ± 2.5 | 15.7 ± 2.4 | 194 ± 50 | 265 ± 53 |
| des-[76-94]b | 264 ± 2 | 432 ± 14 | – | – |
Refolding was carried out in the absence and presence of 200 g/l Ficoll 70.
aRefolding kinetics determined during refolding from the fully reduced state.
bRefolding kinetics determined during refolding of purified des-[76-94].