Figure 2.—
Modulation of ubiquitin-dependent proteolysis by proteotoxic stress conditions. (A) Lysates of adult worms carrying sur-5∷UbV-GFP treated with (+) or without (−) 3 mm DTT were analyzed by immunoblotting with GFP and tubulin antibodies. (B) Worms carrying sur-5∷UbV-GFP or hsp-4∷GFP were treated without (−) or with 10 mm or 3 mm DTT and analyzed as in A. (C) As in A, but worms were treated with or without 1% ethanol. (D) As A, but worms were treated with or without 100 μm cadmium. (E) As A, but worms were treated with or without 2 mm paraquat. (F) As A, but worms were grown to day 1 of adulthood and subjected to 37° for 2 hr (+) or as control (−) were kept at 20°.