Table 2.
Classification of fluorescence objects in synaptosomal field-of-views
| Striatum |
Cortex |
|||
|---|---|---|---|---|
| DA | FT | DA | FT | |
| Type a. TMRM-positive | 51.0 ± 8.1 | 66.6 ± 1.4 | 57.0 ± 0.4 | 66.2 ± 1.1 |
| Type b. TMRM-negative, high-calcium | 29.8 ± 6.2 | 16.6 ± 1.9 | 25.3 ± 2.9 | 17.9 ± 1.3 |
| Type c. TMRM-negative, low-calcium, MitoTracker-positive | 7.9 ± 1.2 | 7.9 ± 0.4 | 8.5 ± 1.5 | 7.7 ± 0.3 |
| Type d. MitoTracker-positive only | 6.3 ± 0.9 | 7.0 ± 1.3 | 6.2 ± 1.1 | 6.4 ± 0.5 |
| Type e. Low-calcium only | 4.9 ± 1.9 | 1.9 ± 0.4 | 2.9 ± 0.6 | 10.7 ± 0.3 |
Each subpopulation described in Fig. 3 was quantified. DA, Separated dopaminergic synaptosomal fraction; FT, residual flow-through fraction. Data are means ± SE (n = 5 biological replicates, each the average of 2–3 technical replicates).