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. 2011 Apr 29;6(4):e19338. doi: 10.1371/journal.pone.0019338

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Masliah et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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To analyze the sub-cellular distribution of the 9E4 antibody immunohistochemical and ultrastructural analysis was conducted in 9E4-FITC immunized α-syn tg mice. (A–C) Representative confocal image of a brain section from an α-syn tg mouse immunized 9E4-FITC and co-labeled with an antibody against α-syn. Arrows indicate co-localization of the 9E4-FITC signal with α-syn in neuronal granular-like structures. (D–F) Confocal image from an α-syn tg mouse immunized 9E4-FITC and co-labeled with an antibody against LC3. Arrows indicate co-localization of the 9E4-FITC with LC3 in neuronal autophagosome-like structures. (G–I) Confocal image from an α-syn tg mouse immunized 9E4-FITC and co-labeled with an antibody against cathepsin D. Arrows indicate co-localization of the 9E4-FITC with cathepsin-D in neuronal lysosomal-like structuresLC3. (J, K) Representative electron micrographs of sections from an α-syn tg mouse immunized with the 9E4 antibody and immunolabeld with gold-tagged anti-mouse antibody. (L, M) Electron micrographs of sections from an α-syn tg mouse immunized with the control IgG1 antibody and immunolabeld with gold-tagged anti-mouse antibody. (N, O) Representative electron micrographs of sections from a non-tg mouse immunized with the 9E4 antibody and immunolabeld with gold-tagged anti-mouse antibody. No reactivity is observed in lysosomes or autophagosomes. Scale bar (A–I) = 10 µM; (J–O) magnification 25,000x.