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RP-HPLC-ESI-MS-MS (150 × 2 mm C18 column; 3μ; 0.2 ml/min; elution solvent: acetonitrile/methanol = 70/30) chromatogram on the DNPH adducts of (A) d0- and d7-DHCEO standards, (B) SLOS HF in the presence of internal d7-DHCEO standard, and (C) E20 Dhcr7-KO mouse brain in the presence of internal d7-DHCEO standard. The SLOS samples were incubated in DMEM with lipid-deficient serum for 5 days.
